Journal: Antioxidants
Article Title: Cosmosiin Induces Apoptosis in Colorectal Cancer by Inhibiting PD-L1 Expression and Inducing ROS
doi: 10.3390/antiox12122131
Figure Lengend Snippet: Measurement of apoptosis-related protein and the ratio of apoptotic cells in a colon cancer cell line by COS. ( A , B ) Protein expression levels for p-AKT, AKT, p-ERK, ERK, PARP, cleaved-PARP, Bcl-2, BAX, and GAPDH were assessed using Western blot analysis. ( C ) The quantification of apoptotic cells was conducted through FACS analysis, employing PI-Annexin V staining. ( D ) The bar chart represents the cells of the ratio of apoptosis. DLD-1, Hct116, and RKO cells were treated with COS (0, 1.25, 2.5, or 5 μM) for a duration of 2 days. The results are shown as the means and SEMs. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 compared to their respective controls. The experiments were conducted three times independently.
Article Snippet: Antibodies targeting B-cell lymphoma-2 (Bcl-2) and Bcl-2-associated X (BAX) were acquired from Novus Biologicals (#NBP2-34443, #NBP1-88682, Littleton, CO, USA).
Techniques: Expressing, Western Blot, Staining